Industrial sugarcane bagasse pile, a lignocellulose-rich with relatively high temperature and anoxic interior environment, represents a good resource for the discovery of microorganisms, which produce lignocellulosic-degrading enzymes that can function under extreme conditions comparable to those prevailing in commercial industrial processes. In the present study, cellulose-degrading activities of thermophilic anaerobic microorganisms isolated from sugarcane bagasse piles were screened for cellulase activity by culturing on carboxy-methylcellulose (CMC) agar and staining with Gram’s iodine. From a total of 502 anaerobic isolates, 121/502 (24.1%) produced halos zone on CMC agar, all of which exhibited the growth and cellulase activity at temperature range of 37°C - 60°C, and pH range of 4-11. The SB7 isolate with the maximal holos zone and the hydrolysis capacity  value of 20.7 after incubation at 60°C for 48 h, was further characterized and identified as Thermoanaerobacterium sp. based on 16S rRNA gene sequence similarity as well as physiological and biochemical properties. The optimal growth conditions of the SB7 isolate was at 60ºC and pH 7. Maximum enzyme production (72 nmole/min/mL) was observed after 54 h. The SB7 isolate also produced xylanase (285 nmole/min/mL) and amylase (2,065 nmole/min/mL) activities, which are of value in term of industrial enzymes. Thus, the strain SB7 obtained from this study could be of interest to further characterization as a promising source for industrial applications.