Vancomycin-dependent enterococci (VDE) are a subset of vancomycin-resistant enterococci (VRE) which require vancomycin for growth. Since the initial report in 1994, there have been multiple subsequent descriptions of individual clinical cases of colonization or infection caused by these organisms, as well as outbreak reports. This presentation provides an overview of VDE, including clinical settings, risk factors, microbiological characteristics, mechanisms of vancomycin dependence, diagnosis and management.