In the practice of converting red algal biomass into biofuel or valuable biochemicals, the critical step is the decomposition process of the agarose to give fermentable monosugars. In this study, we suggest enzymatic process that cost-efficiently produce biomaterials from red algae using multi-enzyme complex. These multi-enzyme complexes including three microbial chimeric enzymes (cAgaB,cCgkA and cAhgA) are stepwise convert the red algae biomass to fermentable sugars. We constructed chimeric gene containing the catalytic domain of β-agarase (AgaB), neoagaro-biose hydrolase (AhgA) from Zobella galactanivorans and κ- carrageenase (CgkA) from Pseudoalteromonas carrageenovora fused with dockerin domain from Clostridium cellulovorans by overap PCR. The multi-enzyme complex including hydrolytic enzymes were formed with scaffolding protein miniCbpA via the cohesion and dockerin interaction. The multi-enzyme complexes were identified using non-denaturing PAGE analysis. And the hydrolysis process of multi-enzyme complex was monitored by reducing sugar assay. As a results, by assembling, the protein band of multi-enzyme complex appeared as a single band molecular weight of 194 kDa. And we observed the increase of fermentable sugar production by multi-enzyme complex as 3.9-fold higher compared with the corresponding enzymes alone (cAgaB) in agar-carrageenan artificial substrates. Reducing sugar produced by the multi-enzyme complex was average at 17.7% higher produce than single enzymes average in all substrate combinations. Also, a combination of the three enzyme complex in substrate have synergistic effects, either enhancing or increasing the degree of polysaccharide breakdown and sugar release 33.5% compared to isolated other combination complexes. In the light of these result, multi-enzyme complex will facilitate the bioconversion of useful products from red algae biomass which represents inexpensive and simple enzymatic process.