<em>In-vitro</em> antibacterial activity of underutilized plant crude extracts against food-borne pathogens — ASN Events
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  3. In-vitro antibacterial activity of underutilized plant crude extracts against food-borne pathogens

In-vitro antibacterial activity of underutilized plant crude extracts against food-borne pathogens (#407)

Nimsha S Weerakkody 1 , KKDS Ranaweera 2 , GAS Premakumara 3 , Thikshani Somarathna 4
  1. Agricultural and Plantation Engineering , The Open University of Sri Lanka, Nawala, Colombo, Sri Lanka
  2. Department of Food Science and Technology, Faculty of Graduate Studies, University of Sri Jayewardenepura, Nugegoda , Sri Lanka
  3. Industrial Technology Institutes, 396, Bauddhaloka Mw, Colombo, Sri Lanka
  4. Agicultural and Planataion Engineering , The Open University of Sri Lanka, Nawala, Colombo, Sri Lanka

There is emerging research interest to discover new antimicrobials from plant sources for food preservation and control of infectious diseases. The antibacterial activity of underutilized plants “Rankihiriya” (Alpinia malaccensis), Purpurata (Alpinia purpurata), “Harankaha” (Cucurma albiflora Thwaites) and “Kottamba” (Terminalia catappa) were evaluated against twelve food-borne pathogens. Crude hexane extracts of all plant rhizomes were obtained, while T. catappa ethanol extract was obtained from the red peri-carp of fruit. The antibacterial activity was determined using agar disc diffusion assay and broth dilution assay. Gas Chromatograph-Mass Spectrometry analysis was performed to the plant with the strongest antibacterial activity to determine chemical composition. Among the four plant extracts, A.malaccensis extract had significantly (p<0.05) higher diameter of inhibition zone ranging (33±1.29 to 40±0.52 mm) against six Staphylococcus aureus  strains including methicillin resistant S.aureus compared to Listeria monocytogens, Eesterichia coli and Salmonella Typhimurium.Also, T.catappa showed higher diameter of inhibition zone (19± 1.15-20±0.57 mm) against S. aureus strains except S. aureus MSSA SS 21D and other bacteria tested. However, C. albiflora showed the significantly (p<0.05) lowest diameter of inhibition zone for all bacteria strains tested. The minimum inhibitory concentration of the A. malaccensis extract was 0.625 mg/ml, and the minimum bactericidal concentration was 5 mg/ml for all the S.aureus strains tested. Gas Chromatograph-Mass Spectrometry of A. malaccensis extract showed a major unknown compound of 82.87% by Willy Library W9N08 while other compounds were, 1,4 dihydrophenanthrene (6.05%), Trans beta faresene (2.64%) and Farnesol, acetate (2.32%). Even though, the major chemical compound was unknown, its mass spectrum was similar to the mass spectrum of Alpinia galanga. Therefore, we speculate that the, major chemical compound could be 1’1'-acetoxy-chavicol acetate and need to further confirm by Nuclear Magnetic Resonance. This study confirmed that uncharacterized, underutilized crops have potential antibacterial activity against food-borne bacteria.

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