Over the past decades, antimicrobial activity of lactic acid bacteria (LAB) has been studied and used as probiotics, which have protective effect against pathogenic bacteria including Clostridium difficile.  C. difficile is an infectious pathogen that can cause gastrointestinal disorders ranging from mild diarrhea to pseudomembranous colitis. There are concerns on antibiotic uses to eradicate C. difficile; alternative strategies to treat and prevent C. difficile infection are therefore of interest. In this study, the probiotic potential properties of Pediococcus pentosaceus, LAB isolated from Thai fermented sausage called nham, were evaluated and the inhibitory effect against C. difficile was also studied. Neutralized cell-free supernatant of P. pentosaceus showed the inhibitory effect against pathogenic bacteria including C. difficile.  The survival in simulated gastric and intestinal fluids as well as adhesion ability to colonic cells were studied. The results showed that P. pentosaceus could survive under tested gastric fluid at pH 3 for 3 h and under basic (pH 8) condition of intestinal fluid for the following 3 h.  This strain was also able to adhere to colonic cells with logCFU/ml of 3.94 (66%). Our findings suggest that P. pentosaceus isolated from nham would be a good candidate as probiotics against C. difficile.